Translational_Unit

Part:BBa_K4310019:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Cold inducible promoter + Cre recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6697
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1199
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
    Illegal NgoMIV site found at 4061
    Illegal NgoMIV site found at 4091
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1381
    Illegal BsaI site found at 3147
    Illegal BsaI site found at 4378
    Illegal BsaI site found at 7628
    Illegal BsaI.rc site found at 1660
    Illegal BsaI.rc site found at 4195
    Illegal BsaI.rc site found at 4657
    Illegal BsaI.rc site found at 5357
    Illegal BsaI.rc site found at 7907
    Illegal SapI site found at 1845
    Illegal SapI site found at 3098
    Illegal SapI site found at 4884
    Illegal SapI.rc site found at 3083
    Illegal SapI.rc site found at 3132
    Illegal SapI.rc site found at 4918


Design Notes

CAMV 35S: BBa_J428074 from distribution 2022 loxP: BBa_K1680005 Cre: BBa_K4297024 mCherry: BBa_J428079 from distribution 2022 Tnos: BBa_J428082 from distribution 2022 pCnAFP: BBa_K4310004 eGFP: BBa_K431006 Kozak: BBa_K4310003


Source

To make compatible to our assembly, CAMV 35S, mCherry and Tnos are amplified by overhang primers and scars of pCnAFP, loxP, and Cre for golden gate assembly is replaced to ours.

References